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KMID : 0903519790220030145
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Journal of the Korean Society of Agricultural Chemistry and Biotechnology
1979 Volume.22 No. 3 p.145 ~ p.149
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Biokinetics of Carbohydrate and Lipid Metabolism in Normal Laying Hen - Part Ⅲ. Determination of Radiochemical Purity of 14C(U) - Glucose Solution by Liquid Scintillation System Using Glucose Pentaacetate -
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Abstract
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The radiochemical purity of ^(14)C(U)-glucose solution to be injected to normal laying lien was investigated for studying biokinetics of carbohydrate and lipid metabolism. The liquid scintillation counter w-as employed for determining the activity of carbon-14. The barium hydroxide and zinc sulfate were adopted to precipitate the protein in the solution. The glucose content in the solution was observed as 0.912㎎ per ㎖, applying Hultman´s method. The specific activity of ^(14)C(U)-glucose solution was known as 31.3nCi/㎎ glucose. The glucose pentaacetate was synthesized to isolate the pure glucose from the solution. The specific activity of pure glucose was measured as 28.5nCi/㎎ glucose. Therefore, it was known that the radiochemical purity of the solution was 82.7%.
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